Sample requirements

 

 

Cancer-Solid tissue requirements

Investigation

Sample requirements

Result/report available

Lymphoid clonality

Ig gene rearrangement

TCR gene rearrangement

Minimum of 3 FFPE embedded tissue scrolls or 1mL EDTA peripheral blood

*Outsourced to Cambridge

 

 

 

Solid Tumour testing

 

 

 

 

All FFPE tissue sections must be sent with a marked up H+E slide.

 

It is important that sections are cut under conditions that prevent cross contamination from other specimens.

 

Samples with <10% neoplastic nuclei: Send unstained tissue sections (slide mounted) on uncoated slides with a marked up H&E for macrodissection

 

Samples with ≥10% neoplastic nuclei: Send unstained tissue sections (as scrolls) in a clean universal with a marked up H&E

 

EGFR

Send a minimum of 30µm FFPE tissue sectioned at 10µm (3x10µm)

Upto 14 calendar days

BRAF

Send a minimum of 30µm FFPE tissue sectioned at 10µm (3x10µm)

Upto 14 calendar days

(3 working days for Melanomas)

KRAS

Send a minimum of 30µm FFPE tissue sectioned at 10µm (3x10µm)

Upto 14 calendar days

NRAS/BRAF

(colorectal only)

Send a minimum of 30µm FFPE tissue sectioned at 10µm (3x10µm)

Upto 14 calendar days

         

*Note we are currently outsourcing B and T cell clonality analysis to Cambridge please forward all material to NUH laboratory for extraction (FFPE embedded tissue or EDTA peripheral blood)

 

 

 

 

Cancer Haemato-oncology requirements:

 

Investigation

Sample requirements

Result/report available

Acute Myeloid Leukaemia (AML)- rapid analysis/fusion detection*

Minimum  of 3.5mL EDTA-anticoagulated peripheral blood/bone marrow

 

FLT-3 ITD/TKD

Upto 3 working days

NPM1

Upto 3 working days

CBFβ-MYH11 inv(16)

Upto 14 calendar days*

AML1-ETO t(8;21)

 

 

IDH1 and IDH2 mutation testing

(Not included in Paediatric AML)

Minimum  of 1mL EDTA-anticoagulated peripheral blood/bone marrow

Upto 14 calendar days*

 

 

APML-fusion detection

PML-RARA t(15;17)

Minimum  of 3mL EDTA-anticoagulated peripheral blood/bone marrow

Upto 3 working days

 

 

CML- fusion detection

BCR::ABL t(9;22)

Minimum  of 3mL EDTA-anticoagulated peripheral blood/bone marrow

Upto 7 calendar days

CML- MRD monitoring BCR::ABL t(9;22) p210 major transcripts

(note we do not offer MRD testing for minor p190 transcript type)

Minimum of 9mL EDTA-anticoagulated peripheral blood/bone marrow

Upto 14 calendar days*

 

 

Myeloproliferative neoplasms

JAK2, CALR

Minimum  of 1mL EDTA-anticoagulated peripheral blood/bone marrow

Upto 14 calendar days

 

 

Post BMT Chimerism MRD

Minimum  of 1mL EDTA-anticoagulated peripheral blood/bone marrow

Upto 14 calendar days


*For clinical urgency based upon priority clinical needs, a request can be made and a 1 week turnaround time arranged with the Lab.

     

 

Note, it is not necessary to take separate blood tubes for each DNA request.