Sample requirements
|
Investigation |
Sample requirements |
Result/report available |
||
|
Lymphoid clonality Ig gene rearrangement TCR gene rearrangement |
Minimum of 3 FFPE embedded tissue scrolls or 1mL EDTA peripheral blood |
*Outsourced to Cambridge |
||
|
|
|
|
||
|
Solid Tumour testing
|
All FFPE tissue sections must be sent with a marked up H+E slide.
It is important that sections are cut under conditions that prevent cross contamination from other specimens.
Samples with <10% neoplastic nuclei: Send unstained tissue sections (slide mounted) on uncoated slides with a marked up H&E for macrodissection
Samples with ≥10% neoplastic nuclei: Send unstained tissue sections (as scrolls) in a clean universal with a marked up H&E
|
|||
|
EGFR |
Send a minimum of 30µm FFPE tissue sectioned at 10µm (3x10µm) |
Upto 14 calendar days |
||
|
BRAF |
Send a minimum of 30µm FFPE tissue sectioned at 10µm (3x10µm) |
Upto 14 calendar days (3 working days for Melanomas) |
||
|
KRAS |
Send a minimum of 30µm FFPE tissue sectioned at 10µm (3x10µm) |
Upto 14 calendar days |
||
|
NRAS/BRAF (colorectal only) |
Send a minimum of 30µm FFPE tissue sectioned at 10µm (3x10µm) |
Upto 14 calendar days |
||
*Note we are currently outsourcing B and T cell clonality analysis to Cambridge please forward all material to NUH laboratory for extraction (FFPE embedded tissue or EDTA peripheral blood)
|
Investigation |
Sample requirements |
Result/report available |
|
Acute Myeloid Leukaemia (AML)- rapid analysis/fusion detection* |
Minimum of 3.5mL EDTA-anticoagulated peripheral blood/bone marrow |
|
|
FLT-3 ITD/TKD |
Upto 3 working days |
|
|
NPM1 |
Upto 3 working days |
|
|
CBFβ-MYH11 inv(16) |
Upto 14 calendar days* |
|
|
AML1-ETO t(8;21) |
||
|
|
|
|
|
IDH1 and IDH2 mutation testing (Not included in Paediatric AML) |
Minimum of 1mL EDTA-anticoagulated peripheral blood/bone marrow |
Upto 14 calendar days* |
|
|
|
|
|
APML-fusion detection PML-RARA t(15;17) |
Minimum of 3mL EDTA-anticoagulated peripheral blood/bone marrow |
Upto 3 working days |
|
|
|
|
|
CML- fusion detection BCR::ABL t(9;22) |
Minimum of 3mL EDTA-anticoagulated peripheral blood/bone marrow |
Upto 7 calendar days |
|
CML- MRD monitoring BCR::ABL t(9;22) p210 major transcripts (note we do not offer MRD testing for minor p190 transcript type) |
Minimum of 9mL EDTA-anticoagulated peripheral blood/bone marrow |
Upto 14 calendar days* |
|
|
|
|
|
Myeloproliferative neoplasms JAK2, CALR |
Minimum of 1mL EDTA-anticoagulated peripheral blood/bone marrow |
Upto 14 calendar days |
|
|
|
|
|
Post BMT Chimerism MRD |
Minimum of 1mL EDTA-anticoagulated peripheral blood/bone marrow |
Upto 14 calendar days |
*For clinical urgency based upon priority clinical needs, a request can be made and a 1 week turnaround time arranged with the Lab.
Note, it is not necessary to take separate blood tubes for each DNA request.