Strike action will be taking place from 7am Saturday 24 February 2024 to Thursday 28 February 2024. Please continue to attend your appointments unless you are contacted and told otherwise.

The strike action may mean our A&E is a lot busier than usual, which means the waiting times will be longer. If you need help which is not life threatening, please use 111, Pharmacies, GPs or Urgent Treatment Centres. Thank you for your understanding during this period.

Sample requirements

 

Cancer-Solid tissue requirements

It is important that sections are cut under conditions that prevent cross contamination from other specimens.

Investigation

Sample requirements

Result/report available

Lymphoid clonality

Ig gene rearrangement

TCR gene rearrangement

Minimum of 3 FFPE embedded tissue scrolls or 1mL EDTA peripheral blood

*Outsourced to Cambridge

Genexus OPA Panel

Minimum of 50μm FFPE tissue sectioned at 10μm (5x10 μm):

  • Samples with <20% neoplastic nuclei: Send unstained slide mounted tissue sections on uncoated slides with a marked up H&E
  • Samples with ≥20% neoplastic nuclei:  Send unstained tissue sections as scrolls in a clean universal with a H&E
 

*Note we are currently outsourcing B and T cell clonality analysis to Cambridge, please forward all material to NUH laboratory for extraction (FFPE embedded tissue or EDTA peripheral blood).

Cancer Haemato-oncology requirements:

 

Investigation

Sample requirements

Result/report available

Acute Myeloid Leukaemia (AML)- rapid analysis/fusion detection*

Minimum  of 3.5mL EDTA-anticoagulated peripheral blood/bone marrow

 

FLT-3 ITD/TKD

Upto 3 working days

NPM1

Upto 3 working days

CBFβ-MYH11 inv(16)

Upto 14 calendar days*

AML1-ETO t(8;21)

 

 

IDH1 and IDH2 mutation testing

(Not included in Paediatric AML)

Minimum  of 1mL EDTA-anticoagulated peripheral blood/bone marrow

Upto 14 calendar days*

 

 

APML-fusion detection

PML-RARA t(15;17)

Minimum  of 3mL EDTA-anticoagulated peripheral blood/bone marrow

Upto 3 working days

 

 

CML- fusion detection

BCR::ABL t(9;22)

Minimum  of 3mL EDTA-anticoagulated peripheral blood/bone marrow

Upto 7 calendar days

CML- MRD monitoring BCR::ABL t(9;22) p210 major transcripts

(note we do not offer MRD testing for minor p190 transcript type)

Minimum of 9mL EDTA-anticoagulated peripheral blood/bone marrow

Upto 14 calendar days*

 

 

Myeloproliferative neoplasms

JAK2, CALR

Minimum  of 1mL EDTA-anticoagulated peripheral blood/bone marrow

Upto 14 calendar days

 

 

Post BMT Chimerism MRD

Minimum  of 1mL EDTA-anticoagulated peripheral blood/bone marrow

Upto 14 calendar days


*For clinical urgency based upon priority clinical needs, a request can be made and a 1 week turnaround time arranged with the Lab.

     

 

Note, it is not necessary to take separate blood tubes for each DNA request.

Page last updated 17/01/2024. Please note that if printed, information is only valid on the day of printing.